Development of Two PCR-Based Techniques for Detecting Helical and Coccoid Forms of Helicobacter pylori
نویسندگان
چکیده
منابع مشابه
Development of two PCR-based techniques for detecting helical and coccoid forms of Helicobacter pylori.
The primary mode of transmission of Helicobacter pylori, a human pathogen carried by more than half the population worldwide, is still unresolved. Some epidemiological data suggest water as a possible transmission route. H. pylori in the environment transforms into a nonculturable, coccoid form, which frequently results in the failure to detect this bacterium in environmental samples by convent...
متن کاملCoccoid forms of Helicobacter pylori.
To the Editor-Eaton et al. [1] recently described experiments with coccoid Helicobacter pylori in gnotobiotic piglets. This interesting study examined the colonization and the reversion from coccoid to bacillary H pylori within 2 weeks of inoculation. Piglets colonized with bacillary H pylori had more severe gastritis 2 weeks after inoculation than did those sacrificed 1 week after inoculation....
متن کاملComparison of the nucleic acids of helical and coccoid forms of Helicobacter pylori.
The nucleic acids of the helical and coccoid forms of Helicobacter pylori were studied to determine if the coccoid forms are "viable (capable of growing) but nonculturable." Using a reference strain (NCTC 11638) and five clinical strains, the nucleic acid contents, DNA integrity, and results of PCR and reverse transcription-PCR (RT-PCR) were compared for helical H. pylori and coccoid forms indu...
متن کاملMethods for Detecting the Environmental Coccoid Form of Helicobacter pylori
Helicobacter pylori is recognized as the most common pathogen to cause gastritis, peptic and duodenal ulcers, and gastric cancer. The organisms are found in two forms: (1) spiral-shaped bacillus and (2) coccoid. H. pylori coccoid form, generally found in the environment, is the transformed form of the normal spiral-shaped bacillus after exposed to water or adverse environmental conditions such ...
متن کاملcomparison pcr and culture methods for detect coccoid forms of helicobacter pylori
background: helicobacter pylori ( pylori ) was the most common cause of chronic gastritis and was linked to peptic ulcer disease and gastric cancer. environmental factors such as water a reservoir of h.pylori which infect human. a non-culture bacteria in coccoid forms widespread in aquatic environments.the objective of this study was elevating the diagnostic value of pcr and culture methods f...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2004
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.42.8.3613-3619.2004